产品名称 单位 货号
AFT Super LipoRNAiMAX Transfection Reagent 0.25 ml AFT2063-01
【储存条件】
长期保存，请置于4˚C (切勿结冰冻住)。
【产品简介】
AFT Super LipoRNAiMAX转染试剂可在多种细胞类型中获得最高的转染效率，适用于siRNA介导的基因抑制实验。AFT Super LipoRNAiMAX是一种独有的RNAi特异性阳离子脂质体转染试剂，适合将siRNA和miRNA输送至所有细胞类型中。
【产品特点】
1）超高的转染效率，且所需RNAi浓度低，基因抑制效果更好，非特异性效应极少
2）细胞毒性低、易于优化，跨10倍转染试剂浓度范围均显示了低细胞毒性
3）针对miRNA拮抗剂和模拟物具有出色的转染效率
4）可用于多种细胞类型，是适合各种基因抑制实验的最通用方法
5)简单快速的实验方案，可获得稳定且可重复的结果
【实验前准备】
• siRNA or miRNA (10 μM stock)
• Opti-MEM Reduced Serum Medium
• 微量离心管
【操作步骤】
A、AFT Super LipoRNAiMAX Transfection Reagent 需要量（具体方法见后面）
Component 96-well 24-well 6-well
Final siRNA per well 1 pmol 5 pmol 25 pmol
Final LipoRNAiMAX Reagent per well 0.3 μL 1.5 μL 7.5 μL
B、Transfection of siRNA
Reverse transfection is faster to perform than forward transfection and is themethod of choice for high-throughput transfection. Perform reverse transfectionby preparing complexes inside the wells, and then adding cells and medium. Because the cells and siRNA-lipid complexes are prepared on the same day, we recommended using 2.5× more cells than for a regular transfection method.
C、AFT Super LipoRNAiMAX Transfection Reagent Protocol（具体方法）
Transfect cells according to the following table. The transfection is designed for one RNA amount combined with one amount of LipoRNAiMAX. The prepared mix is enough to have triplicates (96-well), duplicates (24-well), and single well (6-well) transfections, and account for pipetting variations. For additional information on scaling your transfection reaction.
